BIOETHANOL PRODUCTION FROM SEAWEED PROCESSING WASTE BY SIMULTANEOUS SACCHARIFICATION AND FERMENTATION (SSF)

Andi Hakim; Ekowati Chasanah; Uju Uju; Joko Santoso

doi:10.15578/squalen.281

BIOETHANOL PRODUCTION FROM SEAWEED PROCESSING WASTE BY SIMULTANEOUS SACCHARIFICATION AND FERMENTATION (SSF)

Andi Hakim, Ekowati Chasanah, Uju Uju, Joko Santoso

Abstract

Seaweed processing waste has been used for bioethanol production through simultaneous saccharification and fermentation (SSF). SSF is commonly used for bioethanol production to shorten the process and to increase the yield of ethanol produced by Trichoderma reesei and Saccharomyces cerevisiae. The aim of this research was to obtain the best concentration of T. reesei and S. cerevisiae to produce bioethanol by SSF. The concentration of T. reesei and S. cerevisiae used was 0 (control), 5, 10, 15 and 20% (v/v). The SSF process was carried out by using shaking incubator at 35 °C and rotation of 150 rpm for 3 days. The untreated and hot water treated seaweed processing waste used in this study have moisture content values of 12.94±0.08% and 15.38±0.19%, ash content values of 16.72±0.08% and 18.39±0.19%, lignin content values of 15.38±0.11% and 12.74±0.38%, and cellulose content values of 26.92±0.57% and 34.57±0.81%, respectively. The result of SSF process of seaweed processing waste showed that different concentrations of T. reesei and S. cerevisiae (control, 5, 10, 15 and 20%) yielded significant effect (p<0.05) on the total reducing sugars and ethanol produced. The Duncan Multiple Range Test (DMRT) showed that the treatment 10% of T. reesei and S. cerevisiae concentration in the seaweed processing waste treated with hot water was the best treatment producing highest yield of ethanol.

Keywords

bioethanol, treatment, seaweed processing waste, SSF

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References

Adini S., Kusdiyantini E., & Budiharjo A. (2015). Production of bioethanol from seaweed and waste for Gracilaria sp. With different saccharification methods. Bioma. 16(2), 65-75.

Andhikawati A. (2014). Screening and utilization of endophytic caps in the manufacture of bioethanol from agar-processing waste. Thesis. Bogor (ID): Bogor Agricultural University.

Anindyawati T. (2009). Prospect of enzyme and lignocellulose waste for bioethanol production. BS. 44(1), 49- 56.

Borines MG., Leon RLD., & Cuello JL. (2013). Bioethanol production from the macroalgae Sargassum spp. BioresourceTechnology. 138, 22-29.

El-Naggar NE., Deraz S., & Khalil A. (2014). Bioethanol production from lignocellulosic feedstocks based on enzymatic hydrolysis: current status and recent development. Biotechnology. 13(1), 1-21.

FAO. (2014). The state of the world fisheries and aquaculture: opportunities and challenges. Food And Agriculture Organization Of The United Nations. Rome.

Kim HM., Wi SG., Jung S., Song Y & Bae HJ. (2015). Efficient approach for bioethanol production from red seaweed Gelidium amansii. Bioresource Technology. 175, 128-134.

Ministry of Marine Affairs and Fisheries. (2014). Analysis of marine and fisheries data in 2014. Data, Statistics and Information Center of the Ministry of Marine Affairs and Fisheries. Jakarta.

Martosuyono P., Judge A., & Fawzya YN. (2015). Chemical pretreatment and enzymatic saccharification of seaweed solid waste. Squalen Bull. of Mar. & Fish Postharvest & Biotech. 10(2), 61-71.

Miller GL. (1959). Use of dinitrosaIicyIic acid reagent for determination of reducing sugar. Analytical Chemistry. 31(3), 426-428.

Nahak S., Nahak G., Pradhan I., & Sahu RK. (2011). Bioethanol from marine algae: a solution to global warming problem. J. Appl. Biol. Sci. 1(4), 74-80.

Nurhayati & Kusumawati R. (2014). Cellulose acetate synthesis from effluent treatment waste. JPB Fisheries. 9(2), 97-107.

Pasanda OSR., Azis A., & Kusuma HS. (2016). Utilization of waste seaweed through pretreatment with liquid hot water method and simultaneous fermentation using Bacteria Clostridium thermocellum. J. Mater. Environ. Sci. 7(7), 2526-2533.

Sari RN., Sugiyono., & Assadad L. (2013). Optimization of hydrolysis and fermentation process time in bioethanol production from industrial processing waste (Gracilaria sp.). JPB Fisheries. 8(2), 133-142.

Stevulova N., Cigasova J., Estokova A., Terpakova E., Geffert A., Kacik F., Singovszka E., & Holub M. (2014). Properties characterization of chemically modified hemp hurds. J. Materials. 7, 8131-8150.

Uju, Wijayanta AT., Goto M., & Kamiya N. (2015). Great potency of seaweed waste biomass from the carrageenan industry for bioethanol production by peracetic acid-ionic liquid pretreatment. J. Biomass and Bioenergy. 89, 63-69.

Wei N., Quarterman J., & Jin Y. (2013). Marine macroalgae: an untapped resource for producing fuels and chemicals. Trends in Biotechnology. 31(2), 70-77.

Wahono SK., Rosyida VT., Darsih C., Diah Pratiwi, Frediansyah A., & Hernawan. (2015). Optimization of simultaneous saccharification and fermentation incubation time using cellulose enzyme for sugarcane bagasse on the second-generation bioethanol production technology. Energy Procedia. 65, 331-336.

Wiratmaja IG., Kusuma IGBW., & Winaya INS. (2011). Making second generation ethanol by utilizing Eucheuma cottonii seaweed waste as raw material. Scientific Journal of Mechanical Engineering Cakra M. 5(1), 75-84.

Zhang W., Lin Y., Zhang Q., Wang X., Wu D., Kong H. (2013). Optimisation of simultaneous saccharification and fermentation of wheat straw for ethanol production. Fuel. 112, 331-337

DOI: https://doi.org/10.15578/squalen.281